The game has the player take on the role of an Initiate as they explore the story of the twin protagonists, Jacob and Evie Frye, both of whom are playable. The two arrive in London in 1868, as the Industrial Revolution reaches its climax, bringing its lot of inequality and poverty in a city controlled by the Templars. To stop their rule, the Fryes will take over the organized crime in an effort to gather an army and free London.